MuF proteins have been reported to have a number of functions in different viruses, such as protecting the ends of viral DNA from nuclease attack when entering a host cell. A muf gene is found just downstream of the portal protein gene ( por), and specifies a MuF (SPP1 gp7) family protein of the longer type. The presence of conserved protein domains and characteristic VIRFAM profiles of virus proteins allowed functional assignments for eight proteins, revealing that the first 27 kb of the Hardycor1 genome carries genes encoding key proteins of caudoviruses, including the large subunit terminase (TerL), portal protein (Por), major capsid protein (Mcp) and tape measure protein (Tmp). An independently described algorithm, VICTOR, calculates similarities of viral genomes based on nucleotide or protein sequences, and in both cases, Hardycor1 was predicted to represent a novel species and novel genus ( Supplementary Table S2). Hardycor1 shows negligible similarity (0–2%) to the other 23 virus genomes and represents a novel species and genus. In this scheme, members of the same species share ≥95% nt similarity, while members of the same genus share more than about 70% nt similarity, although more recently, the ICTV have suggested a threshold of ~50% nt similarity for caudoviruses. Values are calculated using the traditional algorithm recommended by the International Committee on Taxonomy of Viruses (ICTV), Bacterial and Archaeal Viruses Subcommittee. Figure 3 shows a heat map of intergenomic similarities of tailed haloviruses, produced using the VIRIDIC suite of programs. The low nucleotide sequence similarity of Hardycor1 to other tailed haloviruses prevents any meaningful alignment or phylogenetic inferences however, whole genome similarity values are useful to define viral taxa. This gene might play an important role in regulation of the temperate state. The attP sequences of many pleolipoproviruses were found to be embedded in a newly detected coding sequence, split in the provirus state, that spans between genes for integrase and a downstream CxxC-motif protein. volcanii showed little similarity (BLASTn) to known viruses and probably represents a novel virus group. A re-examination of other similarly sequenced, archival virus stocks revealed induced proviruses of Haloferax volcanii, Haloferax gibbonsii and Haloarcula hispanica, three of which were pleolipoviruses. Unexpectedly, the sequenced virus stock HC1 also revealed two induced proviruses of the host: a siphovirus (Humcor1) and a pleolipovirus (Humcor2). No close relationship to other viruses was found using phylogenetic tree reconstructions based on TerL and Mcp. Hardycor1 was predicted to be a siphovirus (VIRFAM). Six caudovirus-like proteins were encoded, including large subunit terminase (TerL), major capsid protein (Mcp) and tape measure protein (Tmp). Only twenty-two of the 53 (41%) predicted proteins were significantly similar to sequences in the NCBI nr protein database (E-value ≤ 10 −15). The genome showed little similarity (BLASTn) to known viruses. DNA from a frozen stock (HC1) was sequenced and the viral genome found to be 45,142 bp of dsDNA, probably having redundant, circularly permuted termini. This announcement contains inside information for the purposes of Article 7 of EU Regulation 596/2014.The virus Hardycor1 was isolated in 1998 and infects the haloarchaeon Halorubrum coriense. The outcome of these discussions are subject to normal commercial negotiations and there can be no certainty that these discussions will lead to any contractual agreements or sales.Ī further announcement will be made in due course. The Company also confirms that it is in early stage discussions with a further potential UK distributor and an online retailer. The Company confirms that, in the normal course of business, it expects to receive its first manufactured stock of its wearable device this month as was referred to in the announcement of 24 November 2016. ("CloudTag" or the "Company")ĬloudTag (CTAG:LN), the company that brings personal monitoring to the wellbeing, fitness and digital health markets, notes the recent movement in the Company's share price and speculation that this relates to electronic correspondence from the Company's CEO which has also been made available on social media.
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